Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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The Molecular Devices AD 340 photometric filter (P/N 6590-0019) is a single-wavelength optical filter centered at 340 nm, supplied as a slide-mounted accessory for multimode microplate readers that accept six-position filter slides. It is intended for absorbance and photometric assays requiring stable, narrowband wavelength selection and reproducible microplate measurements.
- Provides narrowband 340 nm wavelength selection for absorbance assays.
- Slide-mounted thin-film optical filter construction.
- Designed for use with six-position filter slide modules in multimode readers.
- Filter slot dimensions compatible with standard slide limits: excitation 12.2-12.7 mm, emission 17.7-18.0 mm, thickness 5.0-9.0 mm.
- Enables reproducible photometric measurements across microplate assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices Absorbance Filter, 414 nm, 10 nm, FilterMax
The FilterMax absorbance photometric filter provides a narrowband 414 nm detection channel with a 10 nm bandwidth for photometric and absorbance assays on compatible multimode microplate readers. It is an optical replacement accessory intended to restore or extend the absorbance measurement capability of readers that accept replaceable filters.
- Center wavelength 414 nm, bandwidth 10 nm.
- Designed for narrowband absorbance and photometric measurements.
- Intended as a replacement optical filter for compatible multimode microplate readers.
- Supports absorbance-based assays that require a 414 nm detection channel.
- Sold as a single replacement filter for installation by qualified service personnel.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices Photometric Filter, 570 nm, Absorbance, Replacement
AD 340 photometric filter for 570 nm absorbance measurements. This replacement optical filter isolates the 570 nm band for photometric assays, improving wavelength specificity and measurement reliability in absorbance-based detection systems.
- Center wavelength 570 nm for absorbance measurements.
- Designed for photometric wavelength selection in plate readers.
- Helps improve assay specificity and reduce spectral interference.
- Compact optical filter form factor for easy installation and replacement.
- Suitable for routine maintenance and filter replacement in absorbance detection workflows.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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